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others suggest separating by 15-20 minutes minimum
The cartilage particles were digested with collagenase type II (0.2% w/v in BM, Worthington Biochemical, Lakewood, NJ, United States) in a 37C shaker overnight
1 Introduction Berberine (C 20 H 18 NO 4 , IUPAC name: 16,17-dimethoxy-5,7-dioxa-13-azoniapentacyclo [11.8.0.0 2,10 .0 4,8 .0 15,20 ] henicosa-1(13),2,4(8),9,14,16,18,20-octaene, PubChem CID: 2353), an isoquinoline quaternary alkaloid, isolated from different medicinal plants with a molar weight of 336.36 g/mol, including Hydrastis canadensis , Xanthorhiza simplicissima , Berberis aristata , Coptis chinensis , Coptis japonica , etc
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