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Whatever your pain tolerance is, the overall procedure is very tolerable
Materials and Methods GSH/GSSG-Glo Assay (Cat.# V6611) CellTox Green Cytotoxicity Assay (Cat.# G8741) menadione (Sigma, Cat.# M5625) l-Buthionine Sulfoximine (Sigma, Cat.# B2515) digitonin (Sigma, Cat.# D141) HEK-293 cells K-562 cells Note: Cell lines were authenticated using STR genotyping ( GenePrint 10 System, Cat.# B9510)
Moreover, recent studies have demonstrated a possible beneficial potential of metformin administration for cancer, obesity, non-alcoholic fatty liver disease, polycystic ovary syndrome, metabolic syndrome patients [6] and could even have neuroprotective effects in Alzheimers disease type pathology [7]
PMCID: PMC3845706
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