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The fixed embryos were washed in PBS and incubated in 5-bromo-4-chloro-3-indolyl-beta-d-galacto-pyranoside (X-gal, Thermo Fisher) staining solution [10 mg/ml X-gal with 3.6 mM K 4 Fe(CN) 6 and 3.1 mM K 3 Fe(CN) 6 ] at room temperature overnight
10.1016/j.abb.2004.05.022 121 MarutaT.InoueT.NoshiM.TamoiM.YabutaY.YoshimuraK.et al
For any man, sexual health changes are deeply personal and can impact confidence, relationships, and quality of life
DOI: 10.1111/j.1442-200x.2005.01998.x [ Links ] Clark MA, Stein REK, Silver EJ, Khalid S, Fuloria M, Esteban-Cruciani NV
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