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Optimization of culture conditions and medium composition for enhanced glutathione production To further enhance glutathione production, the effects of culture temperature, pH, and medium composition were evaluated using the #14 mutant with improved glutathione content
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2.9 Quantification of intracellular ROS levels SH-SY5Y cells were seeded in six-well plates at a density of 8 10 4 cells per well and cultured for 24 h at 37C in a 5% CO 2 atmosphere
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