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A 2-way-ANOVA followed by Sidaks multiple comparison test assessed both NAC-treatment and IPTB-severity factors for each blood compartment and metabolite, while the non-parametric Kruskal-Wallis test was used to assess metabolite differences between blood compartments in each IPTB and treatment subgroup, followed by the Wilcoxon matched-pairs signed-rank test for multiple comparisons between maternal and paired-cord blood samples

In some cases, the sequential use of different B12 status biomarkers or the calculation of a composite B12 status score (derived from a panel of B12 biomarkers and adjusted for folate status and age) can be used to detect deficient states that may otherwise be overlooked when using a single biomarker approach such as a B12 level (92)

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