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Western blotting (WB), immunohistochemistry (IHC) and Immunofluorescence (IF) are adopted to investigate the hub targets expression and signaling pathway activation (e.g., PI3K-AKT pathway, estrogen receptor pathway)
doi: 10.3390/microorganisms10061108 44 NiuHZhouMZogonaDXingZWuTChenRet al
Cells were then collected and examined using a model LSM700 fluorospectrophotometer (Carl Zeiss, Jena, Germany) at an excitation wavelength of 488 nm and an emission wavelength of 535 nm
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