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Then, tissues were mechanically homogenized by passage through 70 m cell strainers, and the resulting homogenates were serially diluted in sterile PBS
Common Mistakes to Avoid Even with the best intentions, it is easy to make mistakes that limit the effectiveness of your routine
Primary antibodies were used at 1:100 dilution and included Anti-NRF2, Anti-KEAP1, anti-ubiquitin, antiLC3, antiCD86, and anti-CD206
In asthma, oxidative stress can be caused by the weakening of enzymatic and non-enzymatic antioxidant mechanisms that accompany chronic inflammations (72) ( Table 1 )
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