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We also evaluated whether GLP-1R and PKA signalling were involved in the protective effect of metformin in HUVECs

At the protein level, although significance was not reached, a numerical decrease in the level of COX-1 production under both endogenous and IL-1-stimulated cell conditions was observed with GS at a concentration of 2 mM (Figure 2C, 2D)

If the excursion was brief (under 6 hours) and temperature remained below 20C, the peptide may retain partial potency, but this introduces unacceptable variability into experimental protocols

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