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Conditions that affect the gut, like Crohns disease or celiac disease, can damage the intestinal lining, making it difficult for B12 to pass into your bloodstream
Dead cells were detected with a ReadyProbes Cell Viability Imaging Kit Blue/Green (Invitrogen): after the end of experiments, two drops per ml of NucBlue Live and NucGreen Dead reagents were added into each well and incubated for 15/30 min at RT to stain the nuclei of all cells (blue) and detect dead cells (green), respectively
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