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In addition, we showed that J10 efficiently degraded DDX3X in all 6 lung cancer cell lines tested in this study (Fig
Acknowledgements The authors offer sincere gratitude to the Head of the Department of Pharmaceutical Sciences, Dibrugarh University for providing the necessary facilities
Standard ratios: 2mg peptide + 2ml water = 1mg/ml (1000mcg/ml) 5mg peptide + 2ml water = 2.5mg/ml (2500mcg/ml) 5mg peptide + 1ml water = 5mg/ml (5000mcg/ml) 10mg peptide + 2ml water = 5mg/ml (5000mcg/ml) How to choose: More water (2-3ml per vial): Easier to measure small doses accurately Larger injection volumes (0.2-0.3ml) Lasts longer (more injections per vial) Less water (1ml per vial): Higher concentration Smaller injection volumes (0.05-0.1ml) Fewer injections per vial Most people use 2ml of water for 5mg vials
The following day, membranes were washed three times with TBST and incubated with the appropriate horseradish peroxidase (HRP)-conjugated secondary antibody for 1 h at room temperature
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