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Quality filtered 16S rRNA sequences were clustered into operational taxonomic units (OTUs), with 97% similarity, by closed reference OTU-picking using the UCLUST algorithm and GreenGenes reference database (v13.5) as implemented in Quantitative Insights into Microbial Ecology (QIIME versions 1.6 and 1.7) [64]
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For the SURPASS-CVOT emulation (tirzepatide vs dulaglutide), the hazard ratio for MACE was 0.83 (95% CI, 0.691.01) compared with 0.92 (95% CI, 0.831.01) in the trial, satisfying all concordance criteria
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