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Apoptosis analysis Cellular apoptosis was assessed with an Annexin V-FITC apoptosis detection kit (Beyotime) according to the manufacturers instructions

(Occasionally, an acute rupture of the Achilles tendon will be treated with casting of the foot and ankle rather than surgery.) Whether a doctor recommends surgery, and what type of surgery he or she recommends, depends on the nature of the Achilles damage as well as the patient and his or her health, lifestyle, and preferences

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Bariatric surgery can result in 2530% mean WL and long-term weight maintenance, however, it is not scalable at the population level and people may be hesitant to this option due to the perceived risk of postoperative complications [9, 10]