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Ice crystals that form during freezing physically shear peptide bonds, similar to the damage caused by vigorous shaking

Early studies from the late 1990s reported that supplementation at 12 grams per day improved mood and reduced fatigue in patients with major depressive disorder, as evidenced by reductions in Hamilton Depression Rating Scale scores by about 2030% over 48 weeks [5]

Oxidation-sensitive residues Peptides containing cysteine, methionine, or tryptophan are particularly vulnerable to oxidation