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Inject slowly into the peptide vial: Direct the stream of liquid gently down the inside wall of the vial rather than directly onto the lyophilised powder, to minimise foaming and potential denaturation

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Individuals who answered yes to item one and self-injection to item two were coded as 1

Individuals with risk factors for B12 deficiency including strict vegans, those with gastrointestinal conditions, patients taking long-term metformin or proton pump inhibitors, and older adultsshould discuss screening with their GP, even in the absence of obvious symptoms