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Unfused albumin variants or GLP-1 albumin fusions (2 mg/mL) were loaded onto the column in a pH gradient from pH 5.5 to pH 8.8 by injecting a gradual mixture of 20 mM MES/HCl, 140 mM NaCl (Sigma-Aldrich) (pH 5.5) and 20 mM Tris/HCl, 140 mM NaCl (Sigma-Aldrich) (pH 8.8) at a flow rate of 0.5 mL/min for 110 min at room temperature

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Yes constipation from glutathione often presents with secondary symptoms including bloating, abdominal distension, and infrequent bowel movements (fewer than three per week)