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After three washes with PBS, sections were incubated with 4,6-diamidino-2-phenylindol (DAPI, 1:10,000 in PBS) at room temperature for 10 min
For example, spinal cord injuries or conditions like multiple sclerosis can lead to muscle spasms or twitches
It's important to note that while B12 injections are generally safe, they can cause side effects in some people, such as itching, swelling, and redness at the injection site, and in rare cases, more serious allergic reactions
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