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This creates a planning problem most researchers don't anticipate until they're staring at leftover solution approaching its expiration
The enzyme xanthine dehydrogenase (XDH), normally producing NADH, is converted to its oxidase form (XO) through calcium-activated proteases or oxidative modification (Kusano et al., 2023)
Removal of the kanamycin cassette was verified by colony PCR using k1, k2 and gshA primers listed in supplementary table S3
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