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McGuire DK, Busui RP, Deanfield J, et al
Liver samples were thoroughly homogenized for 30 s in 500 L of 20 mM phosphate buffer (equimolar mix of K 2 HPO 4 and KH 2 PO 4 ) and then 400 L were mixed with 1 mL of a 1:1 v/v chloroform/methanol mixture, vortexed and then samples were rotated in a wheel for 60 min at RT for 30 min
Apply gentle pressure with a clean cotton swab or gauze for 510 seconds
0.6% acetic acid solution Required for certain peptides like IGF-1 LR3, IGF-1 DES, and NAD+ that degrade in both bacteriostatic and sterile water
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