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Proteins were further purified by reverse affinity in Ni-NTA followed by gel filtration (Superdex 200 16/60, GE Healthcare)
This will help to dissolve some of the stones and make them easier to pass
sCT, Rat Amylin, Elorilintide, Cagrilintide and NN1213 were used as tool compounds to optimize the HTRF cAMP assay in COS7-CTR, AMY1R, AMY2R, AMY3R monoclonal cells
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