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Randomization and masking Participants were randomized to their intervention sequence, either semaglutide then placebo, or placebo then semaglutide
After which, centrifugation was done at 2000 rpm for 15 min, and the supernatants were collected into a fresh tube, 1 mL from each tube, followed by adding 25 l thio-barbituric acid and 75 l ethylene-diamine-tetra acetic acid
Life Sci 2025;362:123381
All procedures were approved by the University of Washington Institutional Animal Care and Use Committee (IACUC Protocol 409101)
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