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coli LOBSTR [Andersen et al.,2013] cells carrying a pETDuet-1 vector encoding codon optimized, C-terminally His-tagged RagA with a mutation (T21N) that favors the GDP-loaded state [Yang et al.,2020,Kim et al.,2008,Shen et al.,2017] and tagless wildtype (WT) RagC in its state (Addgene: 99664), were grown in Terrific broth (TB) and protein expression induced with an overnight IPTG treatment at 18 C b

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Green vegetables, fortified cereals, and grain products are rich in vitamins like riboflavin which is essential in converting glutathione into its active, antioxidant form

If cloudiness persists after gentle mixing, allow the vial to sit for 510 minutes at room temperature and mix again