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The total human evidence base, as of this writing, consists of the following: 1) a Phase I safety trial of 42 healthy volunteers that was registered in 2015, canceled in 2016, and never published, 2) a retrospective case series of 12 patients with knee pain, no control group, and no validated outcome measure, and 3) a 2025 pilot safety study involving two healthy adults who received intravenous infusions at a single private clinic in Florida

Pathway analysis using the Taverna workflow showed that phosphatidylcholines (lysoPC a C17:0, PC aa C32:1, and PC ae C38:4) and methionine are involved in homocysteine metabolism

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