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Integration of metformin-loaded mesoporous bioactive glass nanoparticles and free metformin into polymer microneedles for transdermal delivery on diabetic rats
Juvenile and adult worms were carefully washed in phenol red free RPMI medium and subsequently incubated in phenol red free RPMI medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and at 37 C in a 5% CO 2 incubator overnight
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These unfolded molecules stick together, forming clumps that reduce the effective dose and can potentially cause injection site reactions
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