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Immunofluorescence analysis of phosphorylated Met on cells EHMES-1 cells (2 10 4 cells per well) were seeded in a 96-well glass bottom plate and cultured in RPMI1640 medium supplemented with 10% FBS for 24 h
Surgery 232 (2), 199201
MALE FERTILITY AND SPERMATOGENESIS Hypogonadotropic Hypogonadism: Steroidogenesis Restoration Serum testosterone: increase from 200 ng/dL above baseline Primary failure: no response (Leydig cell damage) Secondary failure: present response but low baseline Applications: Differentiation between primary and secondary hypogonadism Pre-pubertal Leydig cell reserve evaluation Treatment response prediction 5
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