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228,229,230 Inhibiting xCT The search for strategies and drugs aimed at inducing oxidative stress has revealed xCT to be a suitable targetincreased glutamate efflux through this antiporter is an important process for cells to generate sufficient glutathione to cope with high intracellular ROS levels
After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min
Upregulation of GSTT1 and GSTP1 might have important consequences for TCC growth by providing a reduced cellular environment and inhibition of apoptotic pathways
2008;294(3):L478-L488
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