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Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection
We find that this inhibition is due to residual sulfide present in the thiol persulfide substrate cocktail (Fig
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