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Rotate injection sites with each injection within the same area of the body
Biochim et Biophys Acta
10.1016/j.bbagen.2012.09.008 Biochim Biophys Acta
Vitrified sample preparation and data collection Samples (3L) were applied to glow-discharged Quantifoil R1.2/1.3 Cu/Rh 200 mesh grids (Quantifoil GmbH, Grolbichau, Germany) and were flash frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific, Waltham, Massachusetts, USA) set at 100% humidity and 4C for the prep chamber and 10 s blot time
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