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Bull Exp Biol Med 2002;133:6146
The lysate was cleared by centrifugation and the protein was purified by Ni-His-affinity chromatography in batch mode
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One potential mechanism is the Nrf2-ARE pathway, which induces a phase 2 detoxification response that promotes disruption of Nrf2-Keap protein interactions, translocation of Nrf2 to the nucleus, and regulation of target gene expression through the ARE, enhancing cellular defense against oxidative damage (Lau et al., 2008)
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