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Branching morphogenesis assay RPTEC (2 10 4 cells) were suspended in 400 l Cellmatrix type IA collagen solution (Nitta Gelatin) in a 48-well plate and allowed to gel for 60 min at 37 C, according to the manufacturers instructions

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Surprisingly, inhibition of GSH synthesis by BSO was unable to change the distribution of cellular GSH, showing a cellular glutathione pattern different from the one shown in the DEM-treated cells