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During Sharon B
Reconstitute using sterile bacteriostatic water, adding solvent slowly along the vial wall and swirling gently
For the targeted sulfur metabolite quantification approach, the previously established LC-MS conditions were applied 99 with selected reaction monitoring (MRM) using an Ultimate 3000 UPLC system coupled to a Thermo Finnigan TSQ Quantum equipped with HESI (Thermo Fisher Scientific, Waltham, MA)
Follow-up Repeat broad markers after 6-12 weeks if the protocol continues, symptoms change, or there are concerns about inflammation, infection, bruising, swelling, or recovery quality
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