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doi: 10.1038/s41422-021-00557-y
The cells were lysed by ultrasound for 20 min at 4 C, followed by centrifugation at 12,000 rpm for 15 min to collect the supernatant
Studies have shown that early treatment with resveratrol can significantly reduce hearing thresholds at all frequencies, and can reduce the mRNA expression levels of pro-apoptotic genes Bax and Bak, and increase the mRNA expression level of anti-apoptotic genes Bcl-2 and Bcl-xL, but there was no significant difference in hearing levels between the late treatment group and the control group [114]
Data are presented as the mean SEM
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