Routine blood tests form the cornerstone of post-operative monitoring and should typically include: Full blood count (FBC) to detect anaemia Ferritin, serum iron, and transferrin saturation to assess iron stores Vitamin B12 and folate levels 25-hydroxyvitamin D and parathyroid hormone (PTH) Calcium, phosphate, and magnesium Zinc and selenium (less frequently, but recommended in some protocols) Copper and ceruloplasmin should be checked if anaemia, low white cell count (leucopenia), or unexplained neurological symptoms are not explained by iron, B12, or folate deficiency Liver function tests and lipid profile HbA1c particularly relevant for patients with type 2 diabetes The frequency of testing is typically higher in the first year (every three to six months, per BOMSS schedules) and may reduce to annually once levels are stable
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T cells were cultured in human T cell medium (HTCM) consisting of X-Vivo 15 (Lonza, 04-418Q), 5% human AB serum (Fisher Scientific, NC9310328) and 10 mM neutralized N -acetyl-l-cysteine (Sigma-Aldrich, A9165) supplemented with 30 U per ml IL-2 (PeproTech, 200-02 or BRB preclinical biologics repository, National Institutes of Health (NIH)) at 37 C under 5% CO 2
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