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The supernatant containing viral particles was harvested at 48 and 72 h after transfection, and was filtered through Millex-GP Filter Unit (0.45 m pore size, Millipore)

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Kenneth McCall, published a paper in 2025 finding that compounded GLP-1s were linked to higher rates of adverse effects than non-compounded products between 2018 and 2024, including more abdominal pain, diarrhea, and nausea

83 amino acids Modifications: Arg substitution (GluArg at IGF-1 position 3) for reduced IGFBP-3 affinity