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CD4 + CD25 - and CD4 + CD25 + T cells for transfer were obtained from splenocyte suspensions of SPF Wild type mice using a magnetic bead-based CD4 + T cell-isolation kit or the CD4 + CD25 + -Regulatory T cell-isolation Kit (Miltenyi Biotec) according to the manufacturers instructions
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Diabetes Care (2013) 36(9):248996
Patients with obesity who dont have diabetes deserve access to the dose and formulation designed for their condition
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