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A critical problem faced by the researchers during using this technique is that some amino acids, i.e., histidine, cysteine, and tryptophan, may be gradually leached from the stationary phase due to the weakness of interaction with IDA
doi: 10.1002/iub.1324 34
Maintaining the effects of the treatment may make you dependent on it
However, it is thought that biotin may lead to increased expression of SLC19A3 gene
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