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They were then spun down, removed of their supernatant, and resuspended and washed with several changes of PBS, then washed over night in 5 mL PBS
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The most authoritative and contemporary definition was articulated in 2002 by a joint Food and Agriculture Organization (FAO) and World Health Organization (WHO) expert consultation, which described probiotics as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host (15)
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