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Controls simultaneously received an equivolume of saline (5.0 ml/kg ip) or water only (12 ml/rat/day)

Used needles and syringes should be placed in an FDA-cleared puncture-proof sharps container rather than household trash to prevent needle-stick injuries

Before each injection, the vial may be allowed to sit at room temperature for a few minutes to reduce injection discomfort, but it should be returned to the refrigerator immediately after withdrawing the dose