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Studies recorded measurable decreases in specific inflammatory markers after therapy, indicating the improvements extended beyond subjective perception to actual biological changes
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For cFos studies, two to six sections per mouse at the level of the hypothalamus (bregma 0.46 to 1.06 mm) and hindbrain (bregma 7.2 to 7.76 mm) were counted bilaterally and averaged across sections for each mouse
Mapping these pathways allows researchers to gain a deeper understanding of cellular resilience and structural preservation over multi-day periods
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