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An examination of the sensation to the hand is performed

While subcutaneous injection into the lower limbs may be possible, it is generally not recommended as a preferred injection site due to the possible complications involved including tissue damage and injection site reactions: The skin in the lower limbs is typically thinner and more sensitive than other parts of the body, which can increase the risk of pain, bleeding, and infection

This injection is commonly used to treat Vitamin B12 deficiencies and improve overall well-being

Weigh the tissue pieces and homogenize them in PBS at a ratio of 1:9 (e.g., 1g tissue to 9mL PBS) using a glass homogenizer on ice