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Studies have shown that oxidative stress not only directly damages orbital tissues, but also promotes inflammatory responses and fibrosis by activating various signaling pathways ( Various lifestyle factors contribute to oxidative stress, potentially triggering the development of TAO, including smoking, environmental exposures, and sleep disturbances ( 2- ) into less harmful substances such as hydrogen peroxide (H 2 O 2 ), which are subsequently detoxified by catalase and glutathione peroxidase ( Oxidative stress disrupts cellular redox balance, leading to excessive production of oxidants that initiate harmful oxidative reactions

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If the sample contains insoluble matter, centrifuge or filter (0.22 m or 0.45 m filter) before use