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Pickart published 46 articles in peer-reviewed scientific journals and held 29 patents

While the basic biochemical metabolic pathways (see below) involving glutamate and GABA metabolism were worked out from 1950 and onward it was not until the end of this decade that pioneering electrophysiological studies of spinal cord neurons led to the understanding that glutamate and GABA act as excitatory and inhibitory substances (3, 4)

2.8 16S rRNA sequencing analysis Fecal genomic DNA was extracted using a DNA isolation kit, and its concentration and purity were assessed via NanoDrop 2000 and agarose gel electrophoresis