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Sample volumes were normalized to 35 L with EPPS buffer (200 mM, pH 8), diluted with HPLC grade CH 3 CN (9 L), and then labeled (5 L of 20 g/L per sample) with the corresponding TMTsixplex Isobaric Mass Tag (Themo Scientific, #90064B)

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Choline : Choline is an essential amino acid that is used throughout the body to build cell membranes, maintain healthy muscle and liver function, and produce acetylcholine, a neurotransmitter that regulates mood and is involved in forming memories and controlling muscle contractions