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Most providers transition patients to a higher concentration vial once they pass the 1.0 mg weekly dose
Accuracy matters enormously at higher concentrations
Protein samples (20 g) were separated on NuPage Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes
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