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Epoxide hydrolase and epoxygenase metabolites as therapeutic targets for renal diseases
20 l of Stop Solution (Promega, USA) was added into each well and the microplate was shaken for 10 secs
In contrast, the GPXs are involved in the physiological adjustment of peroxide concentrations in the intracellular and extracellular compartments, and are more versatile, so they can also act as scavengers and as repair enzymes
a , HT1080 OE GFP-GPX4 WT and HT1080 OE GFP-GPX4 R152H were treated with 4 M RSL3, 30 g/ml cycloheximide, and 100 M a-Tocopherol for 0, 2, 4, or 6 hours before Western Blot analysis of GPX4 and GADPH
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