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To test whether -KG regulates YBX1 transcription through 2OGDDs, we treated Huh7 and H1299 cells with the pan-2OGDD inhibitor and iron chelator deferoxamine (DFO) 27
Subsequent work documented prolonged GH and IGF-1 stimulation in Phase 1/2 clinical trials (PMID 16352683) and characterised once-daily administration profiles (PMID 16822960)
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Raised the temperature of the reaction mixture to 25-30C and stirred for 4 hr at the same temperature
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