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Annexin V binds phosphatidylserines that translocate to the outer leaflet of the membrane during early apoptosis 61 , whereas 7-AAD is a membrane-impermeable DNA dye that labels cells with compromised membrane integrity, identifying cells undergoing lytic forms of cell death such as necrosis, secondary necrosis and pyroptosis 62,63
In an oxidative ER environment, activated ERO1 generates disulfide bonds by consuming O 2 in the presence of flavin cofactors, which are then passed to the protein for folding via PDI (Wang et al., 2014)
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Li B, Mao J, Wu J, Mao K, Jia Y, Chen F, et al
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