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The eluates containing CPT1B and TEX44-FL were concentrated and filtered through a 30 kDa molecular weight cut-off centrifugal filter unit (Merck Millipore), while the TEX44-C eluate was concentrated and filtered through a 10 kDa unit (Merck Millipore)
Although the pathogenesis of PCOS has not been fully elucidated, recent studies have indicated that excess levels of androgens might be the main pathogenic causative factor of PCOS (144146)
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