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Protein (50 g) was separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis on a Precast PAGE Gel (412%, APPLYGEN, Beijing, China) and electroblotted onto nitrocellulose membranes

Even though our data show that both agents separately caused cell damage, ethanol potentiated the mucosal injury caused by TNBS, an event that was accompanied by a significant depletion of mucosal GSH levels, suggesting that GSH limitation contributed to enhanced cell injury (Table 1)

However, chronic inflammation can contribute to the development of chronic diseases such as arthritis, diabetes, and heart disease

Maintaining a healthy lifestyle, including proper diet and hydration, can enhance the effectiveness of the treatment