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380056) from 6 to 18 weeks of age, and co-housed with counterparts on the same diet, were purchased from The Jackson Laboratory (Bar Harbor, ME, USA)

ICH Q2(R2), for example, describes validation concepts for analytical procedures and includes principles such as specificity, accuracy, precision, and suitable reference materials in analytical validation contexts [2]

For NNMT assays remember that the compound competes at the nicotinamide substrate site, so the apparent potency depends on the nicotinamide concentration used