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To stop any bleeding, apply pressure with gauze or a cotton ball
Alternatively, 2 mL gives 2.5 mg/mL, making a 2.5 mg dose equal to 1 mL, the full syringe
For complete injection technique, see the peptide injection guide
Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development
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